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MOTS-c is a mitochondrial-derived peptide that activates AMPK through direct mitochondrial signaling and nuclear translocation under metabolic stress, whereas metformin activates AMPK indirectly by inhibiting Complex I of the mitochondrial electron transport chain, and AICAR mimics AMP to trick the cell into AMPK activation

Anemia can also be caused by a restricted diet, poor iron absorption, repeated blood testing, or the hemodialysis removal of iron and vitamins

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DNA was not isolated from the first two samples collected in the LD/LD-V group, and the first sample collected in each of the other respective groups, due to the use of saline to flush the cecum (after weighing) during early collection (therefore, n = 25 cecal samples)