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For the reduction of roGFP2, 2 M diamide oxidized roGFP2 WT or 2 M glutathionylated roGFP2 C151S or roGFP2 C208S in syringe 1 were mixed with variable concentrations of reduced PfGrx and GSH with 0.4 U/mL GR and 0.25 mM NADPH in syringe 2

Consequently, modulation of these shared nodes (whether through iron chelation, GSH restoration, GPX4 stabilization, or lipid peroxidation inhibition) may offer broad-spectrum therapeutic strategies, providing a theoretical foundation for developing pan-pathogenic antimicrobial agents

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