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At 7 dpi, the wells were fixed with 2 mL/well of 7% Formaldehyde (VWR,10790-708) for 30 min and with 0.5 mL/well of 1x crystal violet (VWR, 470337-534) for 15 min

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Interventions: Deep diaphragmatic breathing (5-10 minutes before meals) Vagal nerve stimulation (gargling, humming, cold exposure) Adaptogenic herbs (ashwagandha, rhodiola) if stress is chronic When to Worry: Red Flags While constipation is common, certain symptoms require prompt medical attention: Unexplained weight loss Blood in stool Severe bloating or pain Vomiting Constipation lasting more than 2 weeks despite intervention Consult your provider if any of these are present

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