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Any animal-model protocol should include defined humane endpoints, veterinary oversight, and IACUC or equivalent ethics-committee approval before initiation

Getting injections too frequently can complicate symptom tracking and follow-up decisions

The duration of use varies, but many follow a 2- to 4-week cycle for acute injuries, while chronic conditions may require longer use

Neurotoxicology 53 , 148152 (2016)

An Agilent ZORBAX Eclipse Plus C18 (2.1 mm 50 mm, 1.8 m) was used as an analytical column, and an Agilent ZORBAX StableBond-C8 (2.1 mm 5 mm, 1.8 m) (Agilent, Santa Clara, CA, USA) was used as a guard column