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Standard / Gradual Approach (3 mL = ~6,667 mcg/mL) Reconstitute: Add 3.0 mL bacteriostatic water to one 20 mg blend vial ~6,667 mcg/mL total blend (a 10 mg vial ~3,333 mcg/mL)

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Low affinity results in poor yields since the target molecules might leak from the column or are washed away