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Results accelerate in months 23 as your body adapts and you settle into consistent eating patterns

Changes in amyloid-beta and tau in the cerebrospinal fluid of transgenic mice overexpressing amyloid precursor protein

Following primary antibody incubation, sections were rinsed several times in 0.02M KPBS, incubated for 2 hr at room temperature in blocking buffer containing secondary antibodies against rabbit (raised in goat) conjugated with Alexa-Fluor fluorochromes (Life Technologies, Carlsbad, CA) and coverslipped using ProLong mounting medium (Life Technologies, Carlsbad, CA)

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