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This can leave mitochondrial membranes, enzymes and DNA more vulnerable to oxidative injury

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Taking advantage of the reversibility of Michael addition reaction, we developed the first reversible reaction-based fluorescent probe ThiolQuant Green to perform single-point quantification of GSH in living cells 15

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This process is associated with morphological alterations typical for apoptosis, like cell shrinkage, membrane blebbing, and phosphatidylserine (PS) translocation to the outer leaflet of the cell membrane phospholipid bilayer [22, 23]