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Hydrogen/deuterium exchange mass spectrometry (HDX-MS) The individually purified Sh HTL7, At SMAX1 and Sh MAX2 At ASK1 proteins were dialysed in 20 mM HEPES pH 7.5, 200 mM NaCl, 0.5 mM TCEP buffer and then mixed in equimolar ratios to reach final concentrations of 25 M per protein in 200 L final volume (see Supplementary Data 1)

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Interestingly, there was significant enrichment between genes differentially expressed in COPD or associated with worsening lung function in other datasets and those we found to be associated with regional emphysema severity