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Protein concentration was measured using the Pierce Bicinchoninic assay (Thermo Fisher Scientific), as per the manufacturer's instructions, using BSA as the standard

The tail module of mediator complex associates with the enhancer bound transcription factors while the other modules bind to Pol II and PIC at the promoter to dynamically link the promoter and the enhancer

and opportunistic fungi such as Aspergillus spp .) and a decrease in the abundance of beneficial bacteria (e.g., Bifidobacterium bifidum and Lactobacilli ) 72

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