With the development of molecular biology and genetic engineering technologies, humanized animal models such as transgenic mice, gene knockout mice and chimeric mice have appeared one after another (Mak et al., 2012) and clustered regularly interspaced short palindromic repeats-CRISPR-associated (CRISPR/Cas9) (Zhang et al., 2019)
The present invention relates in a further aspect to a method for isolating a GLP-1 peptide, the method including purification of a GLP-1 peptide from a mixture comprising said peptide and related impurities via a cation exchange chromatography process, the cation exchange chromatography process comprising the step of: separating said GLP-1 peptide and said related impurities of said mixture by elution in a solution comprising an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said peptide has a positive local or overall net charge different from the local or overall positive net charge of said related impurities so as to remove said related impurities
Wegrzynowicz, Endogenous neuro-protectants in ammonia toxicity in the central nervous system: facts and hypotheses, Metab Brain Dis 20(4) (2005) 253-263
Intra-arterial vitamin C prevents endothelial dysfunction caused by ischemia-reperfusion
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