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In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min

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HGF/c-Met and EpCAM cross talking in the Wnt pathway Several lines of evidence suggest that EpCAM and c-Met as signal transducers contribute to Wnt downstream effectors to initiate and develop cancer

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The cost implications add up over a multi-month protocol