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The tight junction modulating agents in present invention can be selected from: (i) Phospholipid surfactants, including dodecylphosphocholine (DPC), 1,2-didecyl phosphatidylcholine (DDPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1-didecanoy 1-sn-glycero-3-phospho- choline (LLPC), 1,2-dioctanoyl-sn-glycero-3-phosphocholine (D8PC), 1-1-palmitoyl-2- glutaroyl-sn-glycero-3-phosphocholine (PGPC), the preferred phospholipid surfactants are DPC and DSPC, due to the better solubility and stability in liquid formulations, as well as low mucosal toxicity