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Supernatants were collected and the protein concentrations were determined using a BCA assay kit (Pierce/Thermo Scientific, Rockford, IL) for Western blot analysis and high performance liquid chromatography (HPLC) assay

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Therefore, both excessive activation and inhibition of Nrf2 may be detrimental to DOX-induced cardiomyopathy

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For most peptides, the PK and PD timescales are tightly linked: when serum concentration falls, effect diminishes