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Before in vitro infection, PBMCs were rested overnight in the 24-well plate in a cell incubator at 37C with 5% CO 2

In conclusion, the role of betaine in rodents with NAFLD seems to involve multiple metabolic pathways, and its important role is to regulate the expression of genes involved in fatty acid and lipid metabolism, thus improving the development of liver steatosis

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Yao, C.-c et al

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