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Each time you freeze a peptide solution, ice crystals form

To mimic the trimerized conformation of the native SARS-CoV-2 spike glycoprotein more closely, they fused naturally trimerized human resistin to the spike ectodomain to generate a stable trimer protein antigen called SmT1

Wipe both the peptide vial stopper and the bacteriostatic water vial with alcohol swabs

Gordon Smith A, Robinson Singleton J

The extracellular acidification rate (ECAR), a measure of glycolytic activity, of uninfected primary CD4 + T cells was increased 4-fold following addition of glucose compared to a 6-fold increase in HIV-1 infected cells ( Furthermore, inhibiting glycolysis with the competitive glucose-6-phosphate isomerase inhibitor, 2-DG, also reduces HIV-1 virion production (Valle-Casuso et al., 2019)