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The exposure of ES2 cells to two experimental cycles revealed a significant decrease in total cell death levels in all conditions from the first to the second cycle ( p = 0.000 for N, NC and HC), with the exception of H without cysteine (Fig

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88 In these experiments, we identified a window where drug metabolism and NAPQI formation were completed, but GSH could still effectively protect due to its scavenging of peroxynitrite, suggesting that this oxidant is critical for APAP-induced liver injury