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The effect of 4-week aerobic exercise and dietary control on serum IGF-1 and IGF-1 binding protein-3 levels in obese female adolescents

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The bound ligand was separated from free ligand by filtration through a Perkin Elmer Unifilter-96 GF/C 96-well microplate using the Perkin Elmer Unifilter-96 Harvester (Perkin Elmer, Waltham, MA), followed by three washes with ice-cold assay buffer