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Depletion and other background things as you mention are possible, as well as the B-Vits triggering some detox that has been waiting

Topical and intraperitoneal addition of TB4 increased re-epithelialisation by 42% over saline controls at day 4, and by up to 61% at day 7 post-wounding

Leng, X

That decline is part of why it's drawn research interest in the first place: if a molecule occurs naturally and its levels drop as tissue repair capacity drops, it becomes a reasonable candidate for studying repair signaling directly

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