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Toxicology of paraquat and pharmacology of the protective effect of 5-hydroxy-1-methylhydantoin on lung injury caused by paraquat based on metabolomics

A hundred l PreScission protease (GE Healthcare Life Sciences) solution containing 50 mM Tris (pH 8.0), 100 mM NaCl, 1 mM EDTA, 1 mM DTT was added to the column and incubated for 12~16 hr at 4 C, and then 3MST, which had been excised from GST-fusion, was recovered by centrifugation at 735 g for 1 min at room temperature

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In support of this notion, deletion of HO-1 abrogated oxidative stress-induced mitochondrial iron overload

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