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Polypharmacy associated with AAS and PCT drugs may have serious harmful effects on those engaged in such practices, which constitutes a serious public health concern (Sagoe et al., 2015)

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Tumors were harvested 12 days after initial tumor challenge and dissociated in dissociation buffer rotating at 37C for 30 minutes, passed through a 70 M filter, treated with ACK lysis buffer (ThermoFisher, #A1049201), quenched with RPMI and 10% FBS, and washed in sterile PBS