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TB-500 (synthetic TB4) has been clinically tested for its wound healing potential, with the following two studies worth noting: In a randomized, double-blind, placebo-controlled study with dose escalation, patients suffering from venous stasis ulcers were administered synthetic TB4 locally over a period of 84 days

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Further investigations can include a validation study of the genes involved in the skin irritation mechanism following direct treatment to cells

Beads were pelleted by centrifugation at 12,000 g for 10 s and then washed three times in RIPA buffer