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As to the K-562 cells, cells were collected directly, washed twice with 500 L 1 PBS, and resuspended in 500 L binding buffer
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Activation of nuclear factor-B (NF-B) induces transcription of target genes that primarily encode survival proteins (e.g., cellular FLICE-like inhibitory protein and inhibitor of apoptosis proteins) via formation of the complex I signalosome after binding to TNF receptor-1
Tsutamoto S, Iwasaki Y, Shinohara A, Imamiya R, Samukawa K, Kawada-Matsuo M, et al
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