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Liu J, Zhu S, Zeng L, Li J, Klionsky DJ, Kroemer G, et al

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

10.1136/gut.44.1.81 [DOI] [PMC free article] [PubMed] [Google Scholar] 5.Xu J, Xiao X, Li Y, Zheng J, Li W, Zhang Q, et al

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The performance tests that are unique or most critical to nasal sprays are: (1) Spray Content Uniformity (SCU)/Single Actuation Content (SAC) verifying consistent dose delivery throughout container life per USP