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To determine whether these changes in gene and protein expression led to a functional disruption of the tight junction, immunofluorescence against claudin-5 was performed, demonstrating staining localized to the cell membrane of basal cells
This line of work later led to a separate, FDA-reviewed compound, bremelanotide, which you can read about on our PT-141 page
For a detailed understanding of the key regulatory factors in the ferroptosis-related pathway, including GPX4, System Xc-, NRF2, ACSL4, Coenzyme Q10, FSP1, and p53, refer to Fig
Thangavel C, Perepelyuk M, Boopathi E, Liu Y, Polischak S, Deshpande DA, et al
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