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Before the stimulation, 15 l of NbLumSyt1-HALO was premixed with 3 l of Janelia Fluor 646 HaloTag ligand (100 pmol of JF646 stock at 33 M, Promega GA112A) for 15 min at RT and diluted to 300 l of final volume in Tyrode (to reach 4.85 M final concentration of NbLumSyt1-HALO-JF646)

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For GI-focused use cases, oral delivery may appear more intuitive because the compound passes directly through the digestive tract and makes contact with the tissue it was originally studied in relation to