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The top layer forming the erythrocyte lysate was stored at -80C until further analysis

doi: 10.1186/s12889-021-11270-1 20 SchrderDSchmachtenbergTHeinemannSMllenmeisterCRoderSEl-SayedIet al

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Relevant NICE guidance includes NG28 (Type 2 diabetes in adults: management) and the relevant NICE technology appraisals for these agents